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標題Title: Multiple molecular markers as predictors of colorectal cancer in patients with normal peri-operative serum CEA levels
作者Authors: 溫義輝,Wang JY..等
上傳單位Department: 生物科技系
上傳時間Date: 2009-11-20
上傳者Author: 溫義輝
審核單位Department: 生物科技系
審核老師Teacher: 溫義輝
檔案類型Categories: 論文Thesis
關鍵詞Keyword: predictors, colorectal cancer, serum CEA
摘要Abstract: Purpose: In this study, a high-sensitivity colorimetric membrane array method was used to
detect circulating tumor cells (CTC) in the peripheral blood of colorectal cancer (CRC) patients
with normal perioperative serum carcinoembryonic antigen (CEA) levels. This membrane array
method was evaluated as a potential diagnostic and postoperative surveillance tool.
Study Design: Membrane arrays consisting of a panel of mRNA markers that include human
telomerase reverse transcriptase, cytokeratin-19, cytokeratin-20, and CEA mRNA were used to
detect CTCs in the peripheral blood of157 postoperative CRC patients with normal perioperative
serum CEA levels and in 80 healthy individuals. Digoxigenin-labeled cDNA were amplified by reverse
transcription-PCR from the peripheral blood samples, which were then hybridized to the
membrane array. The sensitivity, specificity, and accuracy of membrane arrays for the detection
of CTCs were then calculated.
Results: Using the four markers in combination, expression of any three markers or all the four
markers in this panel was significantly correlated with the clinicopathologic characteristics,
including depth of tumor invasion, lymph node metastasis, tumor-node-metastasis stage,
and postoperative relapse (all P < 0.05). The interval between the detection of all four positive
molecular markers and subsequent elevated CEA ranged from 3 to 8 months (median 6months).
The expression of all fourmRNAmarkerswas an independent predictor for postoperative relapse.
CRC patients with all four mRNA markers expression showed a significantly poorer survival rate
than those with less than four positive markers.
Conclusions: The constructed membrane array method was helpful in the early prediction of
postoperative relapse in CRC patients with normal perioperative serum CEA levels.

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