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標題Title: DEP CHIP WITH PLANAR MICROELECTRODES ARRAY FOR BIOPARTICLES SEPARATION
作者Authors: 黃舜暉,Cheng-Hsin Chuang..等
上傳單位Department: 機械工程系
上傳時間Date: 2009-11-27
上傳者Author: 黃舜暉
審核單位Department: 機械工程系
審核老師Teacher: 莊承鑫
檔案類型Categories: 文宣Literature
關鍵詞Keyword: Dielectrophoresis, Microfluidic, Separtation, cell
摘要Abstract: This paper presents a microfluidic chip with arc-shape electrode array (ASEA) for separation of different-size particles/Cells based on negative dielectrophoresis (DEP). The DEP chip consists of two parts: first part is a two-channel flow system for focusing particles by a shear flow before enter the ASEA region; the second part is a straight microchannel with several pairs of arc-shape electrodes for generating the dielectrophoretic force perpendicular to the microchannel direction within the each gap between two arc-shape electrodes. The DEP force as a external force for a floating particle, the dielectrophoretic velocity is proportional to the particle size and the gradient of the square of electric field, , from the equilibrium of dielectrophoretic force and viscous drag force under fixed medium conductivity. If the variation of across the microchannel can be reduced by electrode design, the separation effect thus only depends on particle size. In order to construct such condition of electric field, three typically electrode shapes, trapezoidal shape, convex-arc shape and concave-arc shape, were investigated by numerical simulation. As the simulation results, a convex arc-shape electrode can provide better separation effect due to the larger effective area of DEP force and relative small variation of . Currently, the ASEA and SU-8 microchannel have been fabricated by conventional photolithograph. And, the vertical DEP force was experimentally demonstrated by separation of 19 μm and 8μm Latex beads. The separation function will be further examined by 19 μm and 8 μm Latex beads for the practical case of bladder cancer cells and blood cells. This method can provide a continuous and high throughput separation of different-size bioparticles once they have similar dielectric properties that cannot separated by traditional DEP approach.

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2009_11_46bca098.pdf 419Kb pdf 286 106
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