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標題Title： 
DEP CHIP WITH PLANAR MICROELECTRODES ARRAY FOR BIOPARTICLES SEPARATION

作者Authors： 
黃舜暉,ChengHsin Chuang..等

上傳單位Department： 
機械工程系

上傳時間Date： 
20091127

上傳者Author： 
黃舜暉

審核單位Department： 
機械工程系

審核老師Teacher：

莊承鑫

檔案類型Categories： 
文宣Literature

關鍵詞Keyword： 
Dielectrophoresis, Microfluidic, Separtation, cell

摘要Abstract： 
This paper presents a microfluidic chip with arcshape electrode array (ASEA) for separation of differentsize particles/Cells based on negative dielectrophoresis (DEP). The DEP chip consists of two parts: first part is a twochannel flow system for focusing particles by a shear flow before enter the ASEA region; the second part is a straight microchannel with several pairs of arcshape electrodes for generating the dielectrophoretic force perpendicular to the microchannel direction within the each gap between two arcshape electrodes. The DEP force as a external force for a floating particle, the dielectrophoretic velocity is proportional to the particle size and the gradient of the square of electric field, , from the equilibrium of dielectrophoretic force and viscous drag force under fixed medium conductivity. If the variation of across the microchannel can be reduced by electrode design, the separation effect thus only depends on particle size. In order to construct such condition of electric field, three typically electrode shapes, trapezoidal shape, convexarc shape and concavearc shape, were investigated by numerical simulation. As the simulation results, a convex arcshape electrode can provide better separation effect due to the larger effective area of DEP force and relative small variation of . Currently, the ASEA and SU8 microchannel have been fabricated by conventional photolithograph. And, the vertical DEP force was experimentally demonstrated by separation of 19 μm and 8μm Latex beads. The separation function will be further examined by 19 μm and 8 μm Latex beads for the practical case of bladder cancer cells and blood cells. This method can provide a continuous and high throughput separation of differentsize bioparticles once they have similar dielectric properties that cannot separated by traditional DEP approach.

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